miniprotean transblot system (Bio-Rad)

Bio-Rad is a verified supplier

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Bio-Rad miniprotean transblot system
Miniprotean Transblot System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/miniprotean+transblot+system/Mini-PROTEAN+Tetra%2CMini+Trans-Blot+Mod%2CPP+Basic+Power+Supply/pmc05770159-355-36-39
Average 99 stars, based on 83 article reviews

miniprotean transblot system - by Bioz Stars, 2026-09

99/100 stars

Images

SDS Page:

Article Title: Tuning of in vivo cognate B-T cell interactions by Intersectin 2 is required for effective anti-viral B cell immunity
Article Snippet: .. After addition of 2X Laemmli buffer and denaturation at 95 ̊C for 5 min, samples were resolved by SDS-PAGE on precast minigels (7.5% or 12% Tris-HCl; Bio-Rad, Hercules, California), and transferred electrophoretically to PVDF filters using the Miniprotean Transblot system (Bio-Rad). .. Membranes were incubated in WB blocking buffer (5% milk in TBS, 0.1% Tween) to block non-specific binding, and probed with specific Abs diluted in WB blocking buffer or 5% BSA in TBS-T. Primary antibodies used were specific for ITSN2 (Novus, St-Louis, Missouri, RRID:AB_11038593), a-tubulin (Sigma), ERK (Cell Signaling Technology, RRID:AB_330744), phospho-ERK (Cell Signaling Technology, Danvers, Massachussets, RRID:AB_331646), phospho-Akt-S (Cell Signaling Technology, RRID:AB_329825), phospho-SHP1 (Cell Signaling Technology, RRID:AB_11141050), phospho-CD19 (Cell Signaling Technology, RRID:AB_2072836).

Article Title: Tuning of in vivo cognate B-T cell interactions by Intersectin 2 is required for effective anti-viral B cell immunity
Article Snippet: .. After addition of 2X Laemmli buffer and denaturation at 95°C for 5 min, samples were resolved by SDS-PAGE on precast minigels (7.5% or 12% Tris-HCl; Bio-Rad, Hercules, California), and transferred electrophoretically to PVDF filters using the Miniprotean Transblot system (Bio-Rad). .. Membranes were incubated in WB blocking buffer (5% milk in TBS, 0.1% Tween) to block non-specific binding, and probed with specific Abs diluted in WB blocking buffer or 5% BSA in TBS-T. Primary antibodies used were specific for ITSN2 (Novus, St-Louis, Missouri, RRID: AB_11038593 ), α-tubulin (Sigma), ERK (Cell Signaling Technology, RRID: AB_330744 ), phospho-ERK (Cell Signaling Technology, Danvers, Massachussets, RRID: AB_331646 ), phospho-Akt-S (Cell Signaling Technology, RRID: AB_329825 ), phospho-SHP1 (Cell Signaling Technology, RRID: AB_11141050 ), phospho-CD19 (Cell Signaling Technology, RRID: AB_2072836 ).

Review

miniprotean transblot system (Bio-Rad)

Structured Review

Images

Related Articles

SDS Page:

Similar Products

Image Search Results